Effects of Folate Deficiency during Pregnancy on Expression of NKx2.5 Gene in Heart of Offspring
- VernacularTitle:叶酸缺乏对孕鼠子代心脏中NKx2.5基因表达的影响
- Author:
jing, LI
;
shi, LIU
;
yu-zhu, PENG
;
ji-qing, CHEN
;
xi-rong, GUO
- Publication Type:Journal Article
- Keywords:
folic acid;
heart;
embryos;
newborn rats;
NKx2.5 gene
- From:Journal of Applied Clinical Pediatrics
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of NKx2.5 on the heart of offspring during the development of embryo,whose mother is deficient of folic acid.Methods 1.Control group involving 18 rats and study group involving 18 rats were chosen from the total 36 adult female SD rats randomly copulate with the male normal rats after feeding different fodder for 2 weeks.The heart of the 13.5 days,17.5 days embryos and the newborns were obtained;2.the expression of NKx2.5mRNA by RT-PCR was observed;3.the expression of NKx2.5 protein by Western-blotting was investigated.Results 1.The expression of NKx2.5 mRNA of study group was weaker than control group in heart of the 13.5 days,17.5 days embryos and the newborns(P
