Study on Selective Depletion of Alloreactived Lymphocytes by Aminolevulinic Acid-Photodynamic Therapy
- VernacularTitle:氨基乙酰丙酸光动力疗法对同种异体激活淋巴细胞选择性清除作用的研究
- Author:
yi-fei, WANG
;
kai-li, PAN
;
xun, JIANG
;
hai-hong, RAN
- Publication Type:Journal Article
- Keywords:
therapeutics,photodynamic therapy;
leukocyte;
lymphocyte;
photosensitizing agent;
aminolevulinic acid
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of aminolevulinic acid-based photodynamic therapy(ALA-PDT) on alloreactived peripheral blood mononuclear cells(PBMCs).Methods Human PBMCs from different healthy donor were collected and mixed in the one-way mixed lymphocyte culture(MLC) for 5 days. The cells were harvested and aminolevulinic acid(ALA) were added into ALA group and ALA+Light group with ultimate concentrations of 0.5 mmol/L,1.0 mmol/L,1.5 mmol/L,2.0 mmol/L and 2.5 mmol/L.After cultured for 2 hours, 4 hours and 6 hours respectively in 37 ℃ 5% carbon dioxide incubator,Light group and ALA+Light group were irradiated by light of 410 nm wavelength for 1 hour.The MLC cells were treated with the former stimulator cells for 48 hours.The survival of stimulator cells were detected using MTT colorimetric assay and the kill rates of treated cells were calculated.Results The kill rate of ALA+Light group on stimulators was apparently lower than those of Light group, ALA group and control group, (33.0?26.5)% vs (87.1?2.2)%,(89.2?2.5)%,(90.3?1.9)%(All P
