Effect of Postasphyxial-Serum in Neonate Inducing Apoptosis of Renal Tubular Cells
- VernacularTitle:新生儿窒息后血清诱导人肾小管细胞凋亡的作用
- Author:
wen-bin, DONG
;
min, CAO
;
ming-yong, WANG
;
cun-liang, DENG
;
feng, CHEN
;
kai-gui, XU
- Publication Type:Journal Article
- Keywords:
infant,newborn;
asphyxia;
serum;
apoptosis;
nuclear factor-?B;
renal injury
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and intracellular signal transduction mechanism in the injury of renal tubular cells induced by postasphyxial-serum in neonate.Methods Human renal proximal tubular cell(HK-2 cell) was used as target cell. The experiment was designed as:control group, asphyxia group ,and pyrrolidine dithiocarbamate (PDTC)blocking group. The attacking concentration of serum was 20%, and the apoptosis rate of HK-2 cells was detected by flow cytometer.Results Compared with controls[(13.3?1.70)%],after being stimulated with postasphyxial-serum, the apoptosis rate of HK-2 cells of asphyxia group [(46.73?3.68)%] and PDTC blocking group [(31.19?2.79)%]were significantly increased(P
