Expression of Adrenomedullin and Its Receptors mRNA in Neonatal Rats with Hypoxic-Ischemic Brain Damage
- VernacularTitle:缺氧缺血性脑损伤新生鼠肾上腺髓质素及其受体mRNA表达的变化
- Author:
dao-zhuo, BI
;
zhi-rong, WAN
- Publication Type:Journal Article
- Keywords:
hypoxic-ischemic encephalopathy;
adrenomedullin;
receptor;
rat,neonatal;
early
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of adrenomedulin(ADM) and its receptors mRNA in newborn rats with hypoxic-ischemic brain damage(HIBD).Methods Eighty 7-day-old Wistar neonatal rats were randomly assigned 10 groups,to the sham- operation group(the left common carotid artery without ligated and no hpoxia environment),HIBD different time points groups(0,2,4,6,8,10,12,24,48 hours).The expression of ADM mRNA was measured in cortex at different time points by the reverse-transcription polymerase chain reaction(RT-PCR).Results Expressions of ADM and its receptors mRNA in neonatal rats with HIBD increased,and after HIBD 2-4 hours,had a significant difference compared with the sharn-operation group,and reached the highest after HIBD 8-10 hours(P
