Relationship of Membrane Glucocorticoid Receptors in Peripheral Blood Lymphocytes from Minimal Change Nephrotic Children and Influence of Glucocorticoid on Apoptosis and Proliferation
- VernacularTitle:微小病变型肾病患儿外周血淋巴细胞糖皮质激素膜受体与激素对其凋亡、增殖的作用
- Author:
xiao-ming, LIU
;
si-guang, LU
;
zheng, WANG
;
ling-yu, XUE
- Publication Type:Journal Article
- Keywords:
nephrosis,lipoid;
lymphocytes;
glucocorticoids;
apoptosis
- From:Journal of Applied Clinical Pediatrics
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of membrane glucocorticoid receptors(mGR), the correlation between mGR and glucocorticoid(GC)'s effects on apoptosis and proliferation of peripheral blood lymphocytes(PBLs) in minimal change nephrotic syn-drome(MCNS)and the influence of GC on mGR. Methods MCNS, nonminimal change NS(NMCNS) and healthy children were involved in this study. Indirect immune fluorescence and flow cytometry were used to examine the percentages of positive mGR lymphocytes; the apoptosis of PBLs was measured by propidium iodide(PI) staining and the proliferation of PBLs was investigated by fa -TdR incorporation. Results 1.mGR expression in MCNS was higher than that in NMCNS and healthy control,but it was reduced after clinical GC therapy(P
