Level of Transforming Growth Factor - ?_1 in Bone Marrow and Expression of Its Receptor III on Megakaryocytes in Children with Idiopathic Thrombocytopenic Purpura
- VernacularTitle:骨髓转化生长因子-?_1与巨核细胞转化生长因子-?_1 Ⅲ型受体变化在特发性血小板减少性紫癜发病中的意义
- Author:
lu, WANG
;
guang-yao, SHENG
;
song-ting, BAI
- Publication Type:Journal Article
- Keywords:
transforming growth factor- ?1;
receptor;
thrombocytopenia;
megakaryocyte
- From:Journal of Applied Clinical Pediatrics
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study changes and effects of transforming growth factor-?1(TGF- ?1) and its receptorIII (TGF-?1 R III) in children with idiopathic thrombocytopenic purpura (ITP). Methods Bone marrow were respectively collected from 28 children with acute idiopathic thrombocytopenic pupura(AITP),16 children with chronic idiopathic thrombocytopenic purpura(CITP) and 20 comparably normal children; Percoll density gradient and immunomagnetic beads methods were used to purify megakaryocytes from bone marrow; ABC- ELISA was used to detect TGF - ?1 in bone marrow; in situ hybridization was used to detect TGF-?1 RIIImR-NA expression on megakaryocytes.Results In AITP and CITP group, the levels of TGF-?1 and TGF-?1 RIIImRNA were significant higher than those in control group(P
