Changes of Surfactant Protein A and D in Bronchoalveolar Lavage Fluid in Human Fetal Lungs during Various Gestational Ages
- VernacularTitle:不同胎龄人胚肺支气管肺泡灌洗液表面活性物质蛋白A、D的变化
- Author:
yue, CHEN
;
jiang, DU
;
zhi-chun, FENG
- Publication Type:Journal Article
- Keywords:
fetal lung;
bronchoalveolar lavage fluid;
surfactant protein A;
surfactant protein D;
human
- From:Journal of Applied Clinical Pediatrics
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the metabolic rules of surfactant protein A and D(SP- A,SP- D )in bronchoalveolar lavage fluid(BALF)in human fetal lungs during gestational ages. Methods BALF with 30 mL saline was performed on clinically collected human fetus with induction of labor by water- bag Their BALF was respectively retrieved [total retrieval rate(85. 6% ? 13 1)% ]for analysis of protein content. The BALF SP - A and SP - D from fetus of various gestational ages or newboms were detected by RPHA and ELISA. Results The total protein in BALF gradually increased since 10th week to newborn peak during lung development. And SP - A and SP D were respectively updated from(0.34 ?0.07 ) ,(0.05?0.01) ng/L to newborn climax[ (6 42 ? 0 36),(1.22 ? 0 13)ng/L] .Conclusions The protein in BALF gradually increases with fetal growth and lung development. SP-A and SP- D may reach prenatal climax and become the main indicator of newborn lung maturity.
