Effects of Iron Chelators on Labile lron Pool and Apoptosis Related Genes Ex pression in K562 Cells
- VernacularTitle:铁剥夺对K562细胞动态铁池的改变及凋亡相关基因表达的影响
- Author:
xue-qiang, WU
;
guo-cun, JIA
;
yi-ming, YANG
;
yu-feng, LIU
;
yu-ren, XI
- Publication Type:Journal Article
- Keywords:
iron;
deferoxamine;
labile iron pool;
apoptosis related genes
- From:Journal of Applied Clinical Pediatrics
2004;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of iron chelators on labile iron pool and expression of apoptosis associated genes in cells of K562, an erythroleukemia cell line.Methods K562 cells were incubated at 37 ℃ in RPMI 1640 containing 10% heat-inactived fetal bovine serum in an saturated humidity and 5% CO_2 incubator. K562 cells were incubated with different concentrations of desferro-(xamine(DFO)). The study groups were divided as following: DFO group, iron+DFO group and the control group. Following indices were detected which included apoptosis by flow cytometry (FCM) assay, expression of Rb, c-myc, bax mRNA by RT-PCR. The intracellular LIP was measured with a fluorimetric assay using the metalsensitive probe calcein-AM.Results 1. The viability of K562 cells incubated with different concentrations of DFO was lower than that of control group at 12 h,24 h and 48 h (P
