Expression of Apoptotic Signal Proteins in Children with Idiopathic T hrombocytopenic Purpura and Its Clinical Significance
- VernacularTitle:特发性血小板减少性紫癜患儿外周血T细胞凋亡信号蛋白表达的临床意义
- Author:
chang-lin, WU
;
fang, LIU
;
hong, XIAO
;
qun, CHEN
;
xiao-meng, YANG
- Publication Type:Journal Article
- Keywords:
idiopathic thrombocytopaenic purpura;
T lymphocytes;
apoptotic signal proteins
- From:Journal of Applied Clinical Pediatrics
2004;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the exprestion of apoptotic signal proteins(FADD,Fas,FasL and NF-?B) in peripheral blood T lymphocytes in idiopathic thrombocytopenic purpura (ITP) children and its correlation with clinical outcome.Methods Collecting aseptic peripheral blood from ITP children (n=35) and healthy children (n=30), T lymphocytes were isolated and purified by the T cell Segregation Enrichment Column, Fas,FasL and T cell apoptosic ratio were detected by FCM. Immunohistochemical staining was used to detect the level of NF-?B and FADD.Results The expression rates of Fas,FADD in ITP children decreased,but the expression rates of FasL,NF-?B increased.The differences between ITP children and heathy children had statistics significance(P
