Effects of Mailuoning to Excitatory Amino Acid and Nitric Oxi de Synthase in Cerebral Cortex of Neonatal Sprague-Dawely Rats after Hypoxia-I schemia
- VernacularTitle:脉络宁对新生鼠缺氧缺血后大脑皮质兴奋性氨基酸和一氧化氮合酶的影响
- Author:
ke-ya, SUN
;
xing-wang, WANG
;
liu-bao, ZHANG
;
zheng-sheng, JIANG
- Publication Type:Journal Article
- Keywords:
mailuoning;
hypoxia-ischemia brain damage;
neonatal rats;
excitatory amino acids;
nitric oxide synthase
- From:Journal of Applied Clinical Pediatrics
2004;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study changes of aspartate(ASP) and glutamaic acid(GLU) in cerebral cortex of neonatal Sprague-Dawely(SD) rats after hypoxia-ischemia and nitric oxide synthase(NOS) immunoactive expression in cerebral neurons were examined to explore mailuoning′s protective effect on hypoxia-ischemia brain damage(HIBD).Methods The HIBD model was established as follows.The right common carotids of the neonatal SD rats 7 days were temporaily ligatured for 1 hour.Then the neonatal SD rats were exposed to 8% oxygen and 92% nitrogen gas mixture for 2 hours. The ASP and GLU were determined in right cerebral cortex using chromatograph,compared with sham-operated group and mailuoning administrated. Ultrastructure changes of neurons in the right cerebral cortex of neonatal SD rats were observed after sham-operated,hypoxia-ischemia and mailuoning administrated using electronmicroscope.Results The level of excitatory amino acid was promoted in right cerebral cortex after hypoxia-ischemia.The volume of excitatory amino acid was reduced sharply mailuoning administrated. Ultrastructure of neurons in the cerebral cortex showed serious injure after hypoxia-ischemia and ultrastructure of neurons in the cerebral cortex appeared slight damage.Conclusion Mailuoning may possess protective effects to the neurons after hypoxia-ischemia through supplying blood to neurons reducing release of excitatory amino acid.
