A Simple and Dependable Approach to Establish Hypoxic-Ischemic Encephalopathy Model in Neonatal Rat
- VernacularTitle:建立新生鼠缺氧缺血性脑病模型的一种简单可靠的方法
- Author:
xiao-juan, YIN
;
rong, JU
;
zhi-chun, FENG
- Publication Type:Journal Article
- Keywords:
neonatal rat;
brain anoxia-ischemia;
animal model
- From:Journal of Applied Clinical Pediatrics
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate a simple and dependable approach to establish the hypoxic-ischemic encephalopathy model in neonatal rat. Methods Twenty-one neonatal rats of 7 days old were randomly divided into control group,hypoxic group,and hypxic-ischemic group.Every group was randomly divided into 3 hours,6 hours,1 day,3 days,7 days, 14 days,and 21 days group,according to the time of killing.Left common carotid artery of neonatal rats at age of 7 days in hypoxic-ischemic group were ligated.Then,the rats in hypoxic and hypoxic-ischemic group were put in a state of 8% oxygen for 2.5 hours. Brain tissues of rats in 3 groups were observed with HE staining under light microscope.Results In hypoxic-ischemic group,there was found mild brain damage after hypoxic-ischomic 3 hours,the brain lesion was most severe at 1 day,glial cell proliferation was found at 3 days,much neur were losed at 14,21 days,and colloid scar was formed in cortex,striatum and hippocampi.Conclusion The method that left common carotid ontery of neonatal rats were ligated and then put in 8% oxygen for 2.5 hours is simple, rapid and dependable, which can be applied widely.
