Effect of brain - derived neurotrophic factor on apoptosis of embryo brain suffered from intrauterine hypoxic - ischemic injury
- VernacularTitle:脑源性神经营养因子对缺氧缺血胚鼠脑细胞凋亡的作用
- Author:
meng, MAO
;
dan, YU
;
jie, ZHANG
;
hui, ZHOU
;
zhi-ling, WANG
- Publication Type:Journal Article
- Keywords:
brain - derived neurotrophic factor;
intrauterine hypoxia;
apoptosis
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and possible signal pathway of brain - derived neurotrophic factor (BDNF) on apoptosis of rat embryo brain cells suffering from intrauterine hypoxic - ischemic injury. Methods The uterine arteries of the pregnant rats were clamped for 30 minutes in both experimental group and control group. BDNF(2 ?g) was injected into rats in experimental group while saline was injected into rats in control group through caudal veins. The samples were collected at 24, 48 and 72 hours re-spectively after artery clamping. Neuroapoptosis of different groups induced by ischemic damage was measured by TUNEL assay. The expression of extracellular signal - regulated kinase(ERK)and c - Jun - N - terminal kinase(JNK) were observed by immunohisloche-mistry.Results The number of apoptosis cells after hypoxic - ischemic injury increased progressively with time.The apoptosis cells number in experimental group were much lower in number than those of ischemic control group.The expression of ERK increased while the expression of JNK decreased in experimental group, comparing with that of the ischemic control group, with statistical signif-icance (P
