Effect of Chinese Herbs on ?-Globin Gene Cluster Locus Control Hepersensitive site 2 Site Binding with Nucleoprotein
- VernacularTitle:中药治疗对?-珠蛋白基因簇位点调控区高敏位点2与核蛋白结合作用的影响
- Author:
li-min, CHAI
;
zhi-kui, WU
;
xin-hua, ZHANG
;
zhi-ang, LIU
;
hui-guo, CAI
- Publication Type:Journal Article
- Keywords:
Chinese herbs;
hepersensitive site 2 site in ?-globin gene;
cluster locus control region;
nucleoprotein;
binding function
- From:Journal of Applied Clinical Pediatrics
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore molecular mechanism and the curative effect of Yisuishengxue powder and its function of the hepersensitive site 2 (HS2) in ?-globin gene cluster locus control region binding with nucleoprotein.Methods After 3 months treatment of Yisuishengxue powder, nucleoprotein was extracted from the morrow cell before and after treatment. The HS2 DNA probes was combined with nucleoproteins.Electrophoresis gel mobile lag was utilized for observing the mobile velocity of DNA segment.Observe the mobile velocity of DNA probes.Results The mobile velocity of probes combined with nucleoproteins before treatment was different form that of the controls, while it was very close to the controls after treatment.Conclusions It is suggested that this compounding medicine might affect the DNA segment of HS2 site in ?-LCR binding with nucleoprotein GATA-1, which may be one molecular mechanism of Chinese herb therapy.
