The Role of Hydrogen Sulfide System on Pulmonary Artery Smooth Muscle Cells Cult ured of Rats in Hypoxia Condition
- VernacularTitle:硫化氢体系在大鼠低氧性肺动脉平滑肌细胞增殖中的意义
- Author:
chun-yu, ZHANG
;
bo-lin, CONG
;
li-min, GONG
;
jun-bao, DU
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
hypoxia;
pulmonary artery smooth mus cle cells
- From:Journal of Applied Clinical Pediatrics
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of hypoxia on cystathionine gamma lyase (CSE) gene expr ession of rat cultured pulmonary artery smooth muscle cells (PASMCs) and the mod ulatory effect of hydrogen sulfide on PASMCs proliferation.Methods Rat′s cultured PASMCs were randomly divided into contr ol group,NaHS group,hypoxia group and hypoxia +NaHS group. Each group contains 6 wells of cells. Hypoxic challenge was performed with oxygen concentr ation≤1%. The cell proliferation rate was measured by 3H-thymidine i ncorporation into DNA of the PASMCs. The expression of CSE mRNA was measured by quantitative competitive reverse-transcription polymerase chain reaction (RT-PCR ).Results For the PASMCs in hypoxia group, the amount of CSE mRN A was down-regulated about 67% (P
