Application of Polymerase Chain Reaction in Checking Human Cytomegalovirus o f Cerebral Palsy Children
- VernacularTitle:聚合酶链反应在检测脑性瘫痪患儿人巨细胞病毒感染中的应用
- Author:
yan-hua, LEI
;
jian-xian, WU
;
yi-hong, CAI
;
ming-li, WANG
- Publication Type:Journal Article
- Keywords:
human cytomegalovirus;
cerebral palsy;
polymerase chai n reaction;
enzyme-linked immuno sorbent assay
- From:Journal of Applied Clinical Pediatrics
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical application and significance of polymerase chain reactio n(PCR) in checking human cytomegalovirus (HCMV)in cerebral palsy children. Methods Collecting urine and serum of 56 cerebral palsy (CP) children,using PCR to detec t CMV DNA from urine,isolate CMV from urine,and indirect enzyme-linked immuno so rbent assay(ELISA) detecting CMV IgM、IgG of serum.Results In 56 cases,53.6%cases were CMV DNA positive,there were 9 cases CMV isolation,o bserving CMV characteristic cytopathic effect (CPE) and the positive value of se rum CMV IgM、IgG was 12.5%,37.5% respectively.The positive value in isolation o f the virus and CMV IgM was 100%,10% corresponding with that of CMV DNA.Comp ared the 2 former with the latter,it was significant(P0.05).Conclusions Using PCR can detect CMV DNA from CP children with CMV infection quickly.It can apply in detecting CMV in CP and provide credible evidence for intervention as f ar as early in children with CP. J Appl Clin Pediatr,2005,20(2):157-159
