Effect of Naloxone of Different Dosage on c-fos Expression in Hippocampus Induced by Repeated Febrile Seizures
- VernacularTitle:纳洛酮对高热惊厥大鼠海马神经元c-fos表达的影响
- Author:
ying, SHAN
;
jiong, QIN
;
zhi-xian, YANG
;
ying, HAN
- Publication Type:Journal Article
- Keywords:
febrile seizure;
c-fos expression;
opioid;
naloxone
- From:Journal of Applied Clinical Pediatrics
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of naloxone on c-fos expression in hippocampus induced by repeated febrile seizures(FS).Methods Warm water induced rat FS model was developed in this study. Each rat was induced 7 febrile seizures with the interval of one day. Naloxone-treated rats and FS control rats received injection of naloxone(1 mg/kg,2 mg/kg) or saline once FS occurrence every 2 day respectively. All rats were sacrificed 24 hours after the last seizure. In hippocampus, c-fos expression distribution and semi-quantitative analysis was determined by immuhischemical staining measure and western-blotting respectively.Results Compared with FS control group, naloxone treatment could significantly relieve c-fos expression in hippocampus induced by repeated FS, mainly in dentate gyrus(DG) and CA3 region. The comparison between 1 mg/kg and 2 mg/kg naloxone-treated group showed that 2 mg/kg naloxone could reduce c-fos positive expression more significantly.Conclusion Naloxone of proper dosage may significantly alleviate c-fos expression after repeated FS ,which further proved its antiepileptic function and also implied that endogenous opioid may be involved in the regulation of c-fos expression during seizure.
