Impact of hydrogen sulfide on tissue inhibitor of metalloproteinase-1 gene expression in pulmonary arteries of rats under hypoxia
- VernacularTitle:硫化氢对低氧大鼠肺动脉金属蛋白酶组织抑制因子-1基因表达的影响
- Author:
ying, SHI
;
jun-bao, DU
;
chun-yu, ZHANG
;
chan-shu, TANG
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
hypoxia;
collagen;
tissue inhibitor of metalloproteinase-1
- From:Journal of Applied Clinical Pediatrics
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanisms by which hydrogen sulfide (H2S) regulates collagen metabolism during hypoxic pulmonary vascular structural remodeling. Methods Wistar rats were randomly divided into control group (n=6), hypoxia group (n = 6) and hypoxia+NaHS group(n= 6) H2S content in plasma was measured with spectrophotometry. The expression of collagen I was detected by immunohistochemistry. The expression of tissue inhibitor of metalloproteinase-1(TIMP-1) mRNA was detected with situ hybridization Results Compared with hypoxia group, H2S content in plasma increased significantly and the expressions of collagen I and TIMP-1 mRNA in pulmonary arteries were down- regulated in hypoxia + NaHS group. The H2S content in plasma negatively correlated with TIMP-I mRNA expression. Conclusions H2S increases the degradation of collagen in small and media pulmonary arteries of rats under hypoxia by decreasing the synthesis of TIMP-1, and therefore, attenuats the collagen remodeling in pulmonary arteries of hypoxic rats.
