Apoptosis and proliferation of peripheral blood lymphocytes from primary nephrotic syndrome children and effects of dexamethasone on them
- VernacularTitle:原发性肾病综合征患儿血淋巴细胞凋亡、增殖及地塞米松对其作用
- Author:
xiao-ming, LIU
;
si-guang, LU
;
ling-yu, XUE
;
ping, JI
- Publication Type:Journal Article
- Keywords:
primary nephrotic syndrome;
peripheral blood lymphocytes;
apoptosis;
proliferation;
dexamethasone
- From:Journal of Applied Clinical Pediatrics
2004;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the apoptosis and proliferation of peripheral blood lymphocytes(PBLs) from primary nephrotic syndrome(PNS) and effects of dexamethasone(Dex) on them.Methods Minimal change NS(MCNS), non-minimal change NS(NMCNS) and healthy children were involved in this study. PBLs were cultured in vitro with Dex or PHA+Dex or without PHA and Dex. Apoptosis of PBLs was measured by propidium iodide(PI) staining; The effects of Dex at different concentrons on PBLs′proliferation were investigated by 3H-TdR incorporation.Results The apoptotic rate of vacuity group in MCNS was lower compared with NMCNS and healthy controls(P
