Quick identification of haemophilus influenza with reverse dot blot
- VernacularTitle:反向点杂交快速检测流感嗜血杆菌
- Author:
de-xin, SHEN
;
zhi-chun, FENG
;
jiang, DU
- Publication Type:Journal Article
- Keywords:
reverse dot blot;
oligonucleotide probe;
haemophilus influenza
- From:Journal of Applied Clinical Pediatrics
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the way of quick identification of haemophilus influenzae with reverse dot blot.Methods Oligonucleotide probe which is specially targeted to 16SrDNA of haemophilus influenzae was designed, then fixed the probe to nylon membranes, and hybridized with the production of gain with the universal primers.Results The universal primers could hybridize the target sequence from common pathogenic bacteria by PCR, and oligonucleotide probe could hybridize with haemophilus influenza specially and could not hybridize with other bacterias. It proved that the probe was of highly speciality.Conclusion Reverse dot blot is a good method of quickly identification of haemophilus influenzae.
