The study of hematopoietic cell reaction to interleukin-15 in children with myelodysplastic syndrome
- VernacularTitle:骨髓增生异常综合征造血前体细胞对白细胞介素15反应的研究
- Author:
han-rong, CHENG
;
ming-zhen, CHEN
;
ri-ling, CHEN
;
de-yuan, ZHENG
;
zhong-lv, YE
- Publication Type:Journal Article
- Keywords:
interleukin-15;
myelodysplastic syndrome;
hemopoietic stem cell;
apoptosis
- From:Journal of Applied Clinical Pediatrics
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate children′s myelodysplastic hematopoietic cells reaction to interleukin (IL)-15.Methods CD 34 + cells in bone marrow from 18 myelodysplast syndrome(MDS) patients were purified by an immunomagnetic beads sorting system. Apoptosis of hematopoietic precursors was assayed by propidium iodine staining and flow cytometric analysis.Results On 8th cultured day,when IL-15 concentration was between 0-100 ng/ml,it could suppress apoptosis of hematopoietic cells in MDS patients in a dose-and- time dependent manner. IL-15 in study group significanthy lower than that of control group.Conclusion IL-15 may partly suppress apoptosis of hematopoietic cells in MDS patients.
