Effects of InterleukIn2, 6 (IL-2,6) on Immune Function in Children With Bronchopneumonia
- VernacularTitle:支气管肺炎与白细胞介素2,6及免疫的关系
- Author:
dong-bo, JIANG
;
ji-gao, SHEN
- Publication Type:Journal Article
- Keywords:
interleukin 2;
interleukin 6;
bronchopneumonia
- From:Journal of Applied Clinical Pediatrics
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective IL-2,6 production by PBMNC (peripheral blood mononucleat cells) were ineaeured with an attempt to illustrate the immune function of children with bronchopneumonia.Methods The biological activities of IL-2,6 were measured by CTLL and CESS cell lines respectively In addition, mitogen-stimulated blastogenic transformation (MSBT) was also measured. The results were analysed statistically. Patients or Other Farticipants T enty-eight cases of children with bronshopneumonia, male, 16,female: 12. Age: 8 months to 7 years, average 3. 9 years. 15 normol controls, male: 8, female 7.Age: 2 to 6 years, average 3 .5 years.Interventions 5~6ml of venous blood was obtaincd, followed PBMNC were centrifugalized by. FicollHypaque. Wash three:times with RPMI-1640 medium. Resuspend cell at 5 ?106/ml. Add 1% PHA and culture for 48h. Horvest supernatant and store at - 20℃.Measurement and Main Rcsults The levels of IL-2 in children with bronchopneumonia and normal children were (CPM) 9 538 + 3 363, 16 152 ? 3 411 (t test, P