Effect of regulation in human umbilical vein endothelia cells treated by microRNA-132
10.3980/j.issn.1672-5123.2016.10.08
- VernacularTitle:microRNA-132在人脐静脉内皮细胞中调控作用的研究
- Author:
Li-Xin, ZHANG
;
Li-Juan, TAO
- Publication Type:Journal Article
- Keywords:
microRNA-132;
peroxisome-proliferator-activated receptor-γ coactivator-1α;
human umbilical vein endothelial cells;
hypoxia
- From:
International Eye Science
2016;16(10):1820-1823
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To evaluate the regulatory effect of microRNA-132 ( miR - 132 ) in human umbilical vein endothelial cell ( HUVEC) .
●METHODS: ln vitro cultured human umbilical vein endothelia cells in hypoxic environment for 6h, then maintained under normal oxygen condition for 3h, 6h, 12h, 24h. miR-132 and peroxisome-proliferator-activated receptor-γ coactivator-1α ( PGC-1α) expression was detected by quantitative Real - time polymerase chain reaction and Western blot analysis. Human umbilical vein endothelial cells transfected miR-132 mimic and miR-132 inhibitor( anti-miR-132 ) were measured by quantitative Real-time polymerase chain reaction and Western blot.
●RESULTS: miR - 132 and PGC - 1α expression was significantly ( P < 0. 01 ) upregulated in the hypoxic environment of cells at 3h compared with the normal oxygen condition. After cells transfection, the hypoxic environment the miR-132 and PGC-1α expression were markedly increased compared with the normal oxygen condition. The cells transfected miR- 132- mimic, the expression of the miR-132 and PGC-1α were higher than that of transfected anti-miR-132 and contrast group ( P<0. 01).
●CONCLUSION:miR-132 level is highly expressed in the HUVEC under hypoxia and may be an effect of regulation for PGC-1α.
