Mechanism of bradykinin on inflammations of retinal pigment epithelium cells
10.3980/j.issn.1672-5123.2016.8.07
- VernacularTitle:缓激肽刺激体外培养的视网膜色素上皮细胞炎症反应的作用机制
- Author:
Wen-Ting, CAI
;
Cheng-Da, REN
;
Qing-Yu, LIU
;
Qing-Quan, WEI
;
Ya-Ru, DU
;
Qian-Yi, WANG
;
Jun-Ling, LIU
;
Meng-Mei, HE
;
Jing, YU
- Publication Type:Journal Article
- Keywords:
bradykinin;
proliferative vitreoretinopathy;
cyclooxygenase;
nitric oxide synthase
- From:
International Eye Science
2016;16(8):1430-1434
- CountryChina
- Language:Chinese
-
Abstract:
Abstract?AIM: To investigate mechanism of bradykinin ( BK) on inflammations of retinal pigment epithelium ( RPE) cells.?METHODS: ARPE -19 cells were cultured in vitro, stimulated by 100nM BK for 24h. Cell morphology changes were observed by microscope, and BK receptor localization was detected through cell immunofluorescence. Changes of Ca2+in BK and BR antagonist stimuli were detected by laser scanning confocal microscopy.The expressions of COX-1, COX-2, eNOS and iNOS protein in control group and BK group were detected by Western Blot.?RESULTS: After the stimulation of BK, there was no significant changes of ARPE-19 cells in morphology.Kinin B1 receptors ( B1R ) and B2 receptors ( B2R ) could be detected in ARPE-19 cells.Compared with control group, Ca2+concentrations significantly increased in BK group; in B1R antagonist group and B2R antagonist group Ca2+concentrations increased less than BK group; B1R and B2R antagonist group showed no obvious changes in Ca2+concentrations.Compared with control group, COX-2 and iNOS protein concentrations were significantly increased in BK group (P<0.001).?CONCLUSION:BK induces the increasing expression of COX-2 and iNOS in the cultured ARPE cells through binding with either B1R or B2R.
