Effect of ginkgolide B on high glucose -induced human lens epithelial cell apoptosis
10.3980/j.issn.1672-5123.2016.7.09
- VernacularTitle:银杏内酯B对高糖诱导人晶状体上皮细胞凋亡的影响
- Author:
Yan-Hong, WANG
;
Tian-Ye, LAN
;
Ting, CHEN
;
Ping, LIU
;
Li-Li, LIN
- Publication Type:Journal Article
- Keywords:
ginkgolide B;
high glucose;
lens epithelial cells;
apoptosis
- From:
International Eye Science
2016;16(7):1242-1244
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of ginkgolide B on high glucose-induced human lens epithelial cell (HLEC) apoptosis.
METHODS: The high glucose-induced HLEC model was established. Different concentrations of ginkgolide B were intervened. Cell viability was assayed by MTT assay, morphology of cell apoptosis was observed by hochest33258 staining. Cell ultrastructural changes were detected by transmission electron microscopy. Expressions of apoptosis - related factors caspses - 3 and caspase-9 were tested by colorimetric detection.
RESULTS: High glucose inhibited the activity of HLECs, induced apoptosis reaction of HLECs, caused high expression of apoptosis factors in cells; while ginkgolide B inhibited the decrease of cell viability induced by high glucose, decreased the HLEC apoptosis and reduced the expression of apoptosis factors Caspase-3 and Caspase-9.CONCLUSION: Ginkgolide B may inhibit the expression of caspses-3 and caspase-9 then effectively inhibited high glucose-induced apoptosis in HLECs.
