Effect of FTY720 inhibiting corneal neovascularition induced by sphingosine 1-phosphate
10.3980/j.issn.1672-5123.2015.7.04
- VernacularTitle:FTY720抑制S1P诱导的角膜新生血管的研究
- Author:
Fan, ZHONG
;
Xiao-Zhen, DING
;
Wei-Zhong, YANG
;
Zong-Yin, GAO
;
Xiao-He, LU
- Publication Type:Journal Article
- Keywords:
corneal neovascularization;
fingolimod;
sphingosine 1 - phosphate;
human umbilical vein endothelial cells
- From:
International Eye Science
2015;(7):1134-1138
- CountryChina
- Language:Chinese
-
Abstract:
AlM: To explore the inhibiting effect of FTY720 on corneal neovascularization ( CNV) of rat.METHODS: MTT assay and cells scratch were adopted to observe hyperplasia of human umbilical vein endothelial cells ( HUVECs ) and cell migration induced by sphingosine-1-phosphate(S1P) after using FTY720 of different concentration. The effect of FTY720 on CNV induced by S1P in a rat corneal micropocket model was detected. 30SD rats were randomly divided into group A, group B and group C with 10 rats per group. S1P and 0μg, 5μg, and 20μg FTY720 controlled-released particles were implanted into the corneal stroma. The growth of CNV and having pathological examination on 12d after the operation was observed. Findings was analyzed by one-way ANOVA.RESULTS: 10, 102 , 103 , and 104 nmol/L FTY720 and HUVECs co-incubate 72h could inhibit cell proliferation (P < 0. 01 ), 24h after the function of 10, 100nmol/L FTY720, it could inhibit S1P-induced cell migration and the ability of restricting cell proliferation and cell migration was enhanced with increasing concentration of FTY720. On 12d, after rat corneal micropocket controlled-release particles was implanted into groups A, B, C, the CNV area were respectively 10. 05±1. 19, 6. 59±0. 95, 2. 70± 0.68mm2(F=145. 155, P<0. 01), group A and group B was statistically different and this was the same case between group B and group C (P<0. 01).CONCLUSlON:FTY720 can inhibit S1P-induced corneal neovascularization.
