Mechanism research of miR - 181 regulating human lens epithelial cell apoptosis
10.3980/j.issn.1672-5123.2015.5.04
- VernacularTitle:miR-181调控人晶状体上皮细胞凋亡的机制研究
- Author:
Yu, QIN
;
Jiang-Yue, ZHAO
;
Wen-Ting, LUO
;
Jing, LI
;
Jia, LIU
;
Jin-Song, ZHANG
- Publication Type:Journal Article
- Keywords:
microRNA;
miR - 181;
cell apoptosis;
cataract
- From:
International Eye Science
2015;(5):759-763
- CountryChina
- Language:Chinese
-
Abstract:
?AlM: To investigate the expression of miR-181 in the lens tissue of cataract and the regulating mechanism of miR-181 on apoptosis of human lens epithelial cell.
?METHODS:Real time q-PCR was used to measure the expression of miR-181 in the anterior lens capsules of age - related cataract and human lens epithelial cell apoptosis model. miR- 181 mimic and inhibitor were transfected using Lipofectamine 2 000 to regulate the expression of miR-181, and then Real time q-PCR was used to verify transfection efficiency. Flow cytometry was used to detect the change of cell apoptosis rate.
? RESULTS: Compared with control group, the expression of miR-181 was significantly higher in both the anterior lens capsules of age-related cataract and human lens epithelial cell apoptosis model; the relative expression of miR-181 in lens epithelial cells transfected with miR-181 mimic was increased, whereas decreased in cells transfected with miR-181 inhibitor;the apoptosis rate of cells transfected with miR - 181 mimic was increased, while reduced in miR-181 inhibitor group. Each result was statistically significant (P<0. 01).
?CONCLUSlON:High expression of miR-181 is detected in anterior lens capsule of age-related cataract. miR-181 might play a certain role in the pathogenesis of cataract via promoting human lens epithelial cell apoptosis. miR-181 probably becomes a new approach for the nonoperative treatment of cataract, but the concrete mechanism still needs to be further studied.
