Study of melatonin on the protective effect of hydrogen peroxide - induced oxidative damage in human lens epithelial cells
10.3980/j.issn.1672-5123.2015.5.05
- VernacularTitle:褪黑素对过氧化氢诱导晶状体上皮细胞氧化损伤的保护作用
- Author:
Tian-Rui, DONG
;
Ping, LIU
;
Jin-Hong, NI
- Publication Type:Journal Article
- Keywords:
melatonin;
human lens epithelial cells;
oxidative damage
- From:
International Eye Science
2015;(5):764-766
- CountryChina
- Language:Chinese
-
Abstract:
?AlM: To investigate theprotective effect of melatonin against hydrogen peroxide ( H2 O2 )-induced oxidative damage to human lens epithelial cells.
?METHODS: Sub-culture human lens epithelial cells preprocessed with different concentrations of melatonin for 12h and then 100 μmol/L H2 O2 for 24h. The impact of melatonin on H2 O2-induced lens epithelial cell viability was detected by MTT assay, rate of apoptosis was detected by flow cytometry instrument and activity of apoptosis-related factors, Caspase-3 and Caspase-9, were detected by colorimetric method.
?RESULTS: MTT assay showed that melatonin had no effect on the activity of lens epithelial cells, and the drug can inhibit the decrease of H2 O2-induced cell activity, as well as flow cytometry showed that melatonin can inhibit H2 O2-induced apoptosis. ln addition, melatonin can also reduce H2 O2-induced Caspase-3 and Caspase-9 activity in lens epithelial cells, and their activity decreased with effect of melatonin along with extending time.
?CONCLUSlON: Melatonin can obviously inhibit H2 O2 -induced apoptosis of human lens epithelial cells, which provide reliable experimental basis for drug on treatment of cataract.
