Establishment of hepatocellular carcinoma model in mouse by interventional ultrasound
10.3877/cma.j.issn.1672-6448.2015.04.016
- VernacularTitle:超声介入法建立小鼠肝细胞癌模型的研究
- Author:
Haichao, YANG
;
Tianxiu, DONG
;
Cunli, GUO
;
Wenjing, ZHU
;
Tingting, AN
;
Xiuhua, YANG
- Publication Type:Journal Article
- Keywords:
Models,animal;
Ultrasonography,interventional;
Carcinoma,hepatocellular;
Mice
- From:
Chinese Journal of Medical Ultrasound (Electronic Edition)
2015;(4):335-338
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the feasibility and stability of hepatocellular carcinoma model established in mouse with the guidance of ultrasound, and to explore the value of ultrasonography in hepatocellular carcinoma model.MethodsThe type of H22 cells, whose concentration was 2×107/ml, were injected into the left liver of 30 mice with the guidance of ultrasound. After injection, the mice were examined by ultrasound on the tenth and twentieth day, and implemented laparotomy on the twentieth day. The tumors were examined pathologically. The formation rate and growth of tumors were evaluated, and the ultrasonic features of tumors were observed.ResultsThe formation rate of implanted tumors was 96.7% (29/30), and the peritumoral blood flow could be detected. The diameters measured by ultrasound and explorative laparotomy were (8.4±0.3) mm and (8.6±0.2) mm, respectively (P>0.05). The pathological examination showed that the tumors were hepatocellular carcinoma.ConclusionEstablishing mouse model of hepatocellular carcinoma by ultrasound-guided is simple and minimally invasive, and it provides an ideal model of high formation rate for the study of hepatocellular carcinoma.
