Bone mesenchymaI stem ceIIs and chondroitinaseABC on photoreceptor apoptosis in degenerated retina of rats
10.3980/j.issn.1672-5123.2015.3.07
- VernacularTitle:BMSCs 联合 ChABC 对视网膜变性大鼠光感受器细胞凋亡的影响
- Author:
Xiang-Rong, ZHENG
;
Lin, LIU
;
Peng-Fen, GAO
- Publication Type:Journal Article
- Keywords:
bone mesenchymal stem cells;
chondroitinaseABC;
retinal degeneration;
apoptosis
- From:
International Eye Science
2015;(3):407-410
- CountryChina
- Language:Chinese
-
Abstract:
· AlM: To study the effect of bone mesenchymal stem cells ( BMSCs ) and chondroitinaseABC ( ChABC ) on photoreceptor apoptosis in the retina of sodium iodate-induced rats.
·METHODS:Forty Sprague Dawley rats ( SD rats) were intraperitoneally injected with NalO3 (30g/L, 100mg/kg) to establish the retinal degeneration models ( postnatal 28d).These rats were devided into 4 groups.Group A was not injected, group B was injected with BMSCs, group C was injected with BMSCs and ChABC, and group D was injected with phosphate buffer saline ( PBS).After 28d, subretinal injection were applied. Hematoxyln - eosinstaining ( HE ) , tunel and immunohistochemistry were performed at 21d after subretinal injection.
· RESULTS: Photoreceptor number and photoreceptor apoptosis rate of B and C groups were more than those of A and D groups, and there was significant difference statistically ( P <0.05 ) . Photoreceptor number and photoreceptor apoptosis rate of group B were compared with those of group C, and there was no statistical significance between B and C groups ( P>0.05 ) .Glial fibrillary acidic protein ( GFAP) was expressed by BMSCs after intraocular injection.
· CONCLUSlON: BMSCs and ChABC injected into subretinal space may alleviate photoreceptor apoptosis so as to protect retinal photoreceptor cells in degenerated rats.
