Effect of pirfenidone on the proliferation of rat corneal stromal cells
10.3980/j.issn.1672-5123.2015.2.04
- VernacularTitle:吡非尼酮对大鼠角膜基质细胞增殖的影响
- Author:
Jun-Jie, CHEN
;
Gong-Fa, WU
;
Jun-Shan, LIN
;
Yu-Ting, ZENG
;
Qi-Ting, HUANG
- Publication Type:Journal Article
- Keywords:
pirfenidone;
corneal stromal cells;
TGF-β1;
proliferation
- From:
International Eye Science
2015;(2):201-204
- CountryChina
- Language:Chinese
-
Abstract:
AlM:To investigate the effects of pirfenidone ( PFD) on the proliferation and transfomring growth factor-β1 ( TGF-β1 ) expression in vitro culture rat corneal stromal cells.METHODS: Corneal stromal cells from 8 to 10wk SD rats were isolated, cultured and treated with different concentrations of PFD 0mg/mL (control group), 0. 15mg/mL (experimental group▏), 0. 3mg/mL (experimental group‖), 1mg/mL (experimental group Ⅲ) for 48h. CCK-8 assay was performed to assess cell proliferation, while immunocytochemistry and Western Blot were used to detect the expression of ki-67 and TGF-β1 expression, respectively. RESULTS: Compared with control group, PFD significantly inhibited the proliferation in a dose -dependent manner ( all P < 0. 05 ), so was protein expression of ki-67. PFD significantly down-regulated the expression of TGF-β1 in a dose-dependent manner (P<0. 05).CONCLUSlON: Pirfenidone can significantly inhibit the proliferation of rat corneal stromal cell by down regulating TGF-β1 expression, therefore, it has potential prospect in lightening the corneal wound healing reaction.
