Effect of exogenous PGC -1α on VEGF expression in human retinal vascular endothelial cells
10.3980/j.issn.1672-5123.2015.2.03
- VernacularTitle:外源性PGC-1α对人视网膜血管内皮细胞VEGF表达的影响
- Author:
Jian, JIANG
;
Xiao-Bo, XIA
;
Lu, ZHANG
- Publication Type:Journal Article
- Keywords:
peroxisome - proliferator - activated receptor-γ coactivator- 1α;
vascular endothelial growth factor;
recombinant proteins;
normoxia;
hypoxia
- From:
International Eye Science
2015;(2):198-200
- CountryChina
- Language:Chinese
-
Abstract:
AlM: To investigate the effect of exogenous peroxisome-proliferator-activated receptor-γcoactivator-1α ( PGC-1α) on vascular endothelial growth factor ( VEGF) expression in human retinal vascular endothelial cells ( HRVEC) .METHODS:Recombinant PGC-1α protein was added to HRVEC, and no recombinant PGC-1α protein was added to HRVEC as control group. After 24h of incubation, two groups of cells were then placed into a normoxic ( 20%O2 ) or hypoxic ( 1% O2 ) environment for another 16h. The expression of VEGF mRNA and protein were detected by real - time PCR, ELlSA and immunofluorescence cytochemistry.RESULTS: VEGF mRNA and protein levels in the cells were significantly increased by recombinant PGC - 1αprotein both under normoxia and hypoxia conditions as compared with control groups (P<0. 01).CONCLUSlON: PGC-1α can upregulate the expression of VEGF in HRVEC under normoxia and hypoxia conditions.
