Effects of Coix seed oil on human retinal capillary endothelial cells proliferation and VEGF expression in high glucose environment
10.3980/j.issn.1672-5123.2014.12.10
- VernacularTitle:薏苡仁油对高糖环境下人视网膜血管内皮细胞增殖和VEGF表达的影响
- Author:
Min, LI
;
Yun-Sheng, ZHANG
;
Fang, LI
;
Hui-Can, PENG
- Publication Type:Journal Article
- Keywords:
Coix seed oil;
vascular endothelial growth factor;
human retinal capillary endothelial cells
- From:
International Eye Science
2014;(12):2147-2150
- CountryChina
- Language:Chinese
-
Abstract:
AlM: To study the effects of different concentrations of Coix seed oil on human retinal capillary endothelial cells ( HRCECs ) proliferation and vascular endothelial growth factor ( VEGF) expression in high glucose environment.
METHODS: HRCECs extracted from human fresher eyeball and cultured in vitro, and ultimately used in the experiment were the growth of 3rd ~ 4th cells, the experimental were divided into blank control group, low glucose control group, high glucose control group, high glucose + ( 50ü L/mL, 100ü L/mL, 200ü L/mL ) different concentrations Coix seed oil group. Detecting the multiplication of HRCECs by MTT, the immunocytochemical method was employed to detect the each group HRCECs of VEGF expression.
RESULTS:MTT assay results showed that: different concentrations of coix seed oil acted at HRCECs for 48h, inhibition of cell proliferation was significant difference compared with high glucose control group (P<0. 05). Within 48h showed concentration dependence. There was no statistical difference between the low glucose group and high glucose control group (P>0. 05). lmmunocytochemical assay showed that:50ü L/mL, 100ü L/mL, 200ü L/mL Coix seed oil acted at HRCECs 48h, the expression of VEGF decreased significantly compared with the high glucose control group ( P< 0. 05 ), and in a dose- dependent manner. However, in high glucose control group, the expression of VEGF was obvious higher than that of low glucose control group (P<0. 05).
CONCLUSlON:Coix seed oil can inhibit the HRCECs proliferation and suppress the VEGF expression in high glucose environment.
