Influence of Hypertonicity on the Phenotype and Function of Bone Marrow-Derived Dendritic Cells.
- Author:
Sun Moon KIM
1
;
Seung Hee YANG
;
Yon Su KIM
Author Information
1. Department of Internal Medicine, Chungbuk National University College of Medicine, Cheongju, Korea.
- Publication Type:Original Article
- Keywords:
Dendritic cells;
NFAT5;
Osmolarity
- MeSH:
Antigen-Presenting Cells;
Carrier Proteins;
Cell Proliferation;
Dendritic Cells;
Flow Cytometry;
Granulocyte-Macrophage Colony-Stimulating Factor;
Interleukin-1;
Lymphocyte Culture Test, Mixed;
Osmolar Concentration;
Phenotype;
T-Lymphocytes;
Tumor Necrosis Factor-alpha;
Up-Regulation
- From:Korean Journal of Nephrology
2010;29(4):427-433
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Dendritic cells (DCs) are considered the most professional antigen-presenting cells (APCs) because of their unique role in initiating immunity against threatening antigens. Recently, hypertonicity has been suggested to be involved in the activation and development of immune cells such as T cells. And tonicity enhancer binding protein (TonEBP) has been thought to play a pivotal role in this process. Here, we studied the maturation status of DCs and expression of TonEBP in DCs exposed to hypertonic condition. METHODS: Murine bone marrow-derived DCs were generated in the presence of GM-CSF for 6 days, and then exposed to hypertonic media. We evaluated the functional capacities and maturation of DCs using flow cytometry, mixed lymphocyte reaction, and cytokine analysis. Also we investigated the expression of TonEBP in DCs cultured in variable hypertonic media. RESULTS: Mild hypertonicity made CD11c+ DCs to have up-regulation of CD40, 80, and 86. DCs exposed to 320 mOsm/kg media stimulated allogeneic T cell proliferation most effectively compared to DCs exposed to other tonic conditions. However, DCs exposed to 400 mOsm/kg media showed similar stimulatory capacities to isotonic control. Consistent with the phenotype changes, IL-1, 6, and TNF-alpha secretion increased in CD11c+ DCs exposed to mild hypertonic condition. Though we confirmed that TonEBP was expressed in CD11c+ DCs, the amount of upregulation was not dependent on the degree of hypertonicity. CONCLUSION: Our results suggest that hypertonicity enhances the maturation and activation of DCs.
