Identification of genotype of chlamydia trachomatis from outpatients of trachoma
10.3760/cma.j.issn.2095-0160.2013.09.012
- VernacularTitle:沙眼患者病原体基因型的鉴定分析
- Author:
Yu-mei, ZHOU
;
Zhi-qun, WANG
;
Ran, LI
;
Xu-guan, SUN
- Publication Type:Journal Article
- Keywords:
Trachoma;
Chlamydia trachomatis;
Deoxyribonucleic acid;
Ribosomal: Genotype;
Nested PCR
- From:
Chinese Journal of Experimental Ophthalmology
2013;31(9):855-858
- CountryChina
- Language:Chinese
-
Abstract:
Background Trachoma is a common disease in ophthalmology,but few reports about the pathogenetic genotype are reported.Objective This study was to investigate the genotypes of Chlamydia trachomatis.Methods Conjunctival specimens were collected in 16 patients with trachoma in Beijing Tongren Eye Center from January,2003 and August,2006.Variable sequence 4 (VS4) of ompl fragment was amplified by nested PCR(n-PCR) using specific primer of Chlamydia trachomatis,and then the PCR products were sequenced.The DNA sequences were analyzed by software Clustal X and MEGA2,and the genetic characteristics were compared with the known sequences of GenBank.Results The PCR product fragment of MOMP gene of trachoma was 277 bp.Three types of Chlamydia trachomatis strains were idcntified in the 16 specimens,including B-genotype in 9 strains (56.3％),C-genotype in 4 strains(25.0％) and D-genotype in 3 strains(18.7％).High homology was seen in the gene sequences of Chlamydia trachomatis strains between the B-genotype or C-genotype strains and the same genotypes of GenBank or those from some districts of China.However,some differences were exhibited among the Dgenotype strains.Conclusions Identification of genotype of Chlamydia trachomatis has differential effect on trachoma and inclusion conjunctivitis.
