Difference analysis of proteome between diabetic cataract and age related cataract
10.3760/cma.j.issn.2095-0160.2012.06.016
- VernacularTitle:糖尿病性白内障和年龄相关性白内障晶状体蛋白质组学的差异分析
- Author:
Qian-qian, YU
;
Yong, YAO
;
Zhao-dong, CHU
;
Yong-hui, TAO
;
Jun, SHAO
;
Yu-zheng, HUANG
- Publication Type:Journal Article
- Keywords:
Crystallins;
Proteomics;
Diabetic cataract;
Two dimensional electrophoresis;
Mass spectrography
- From:
Chinese Journal of Experimental Ophthalmology
2012;30(6):548-552
- CountryChina
- Language:Chinese
-
Abstract:
Background With the changes of diet and living style,the diabetes has become the major diseases affecting human health.Diabetic cataract is a common complication of diabetes. Objective The present study was to investigate the difference of lens proteomics between diabetic cataract and age related cataract using two dimensional electrophoresis (2-DE) and mass spectrometry in order to postpone happening of diabetic cataract and offer the effective approach to the prevention and therapy of diabetic cataract. Methods The lenses were obtained from 8 diabetic patients and 12 age-related cataract patients during the surgery to extract the protein by lysis and centrifugation.The lens proteins were separated using immobilized pH gradients 2-DE.Image analysis was carried out using PDQuest Advanced-8.0.1 software package.Significant difference of the crystallines was identified by matrixassisted laser adsorption/ionization time of-flight-mass spectrometry (MALDI-TOF-MS) and peptide mass fingerprint combined with protein database. Results The maps of 2-DE showed that lens proteins of diabetic cataract and age related cataract were in the section of pH 5-9 with the relative molecular weight 14000-97000;while relative molecular weight of more abundant crystalline was localized at 20000-31000.About 3 differential protein spots were detected by image analysis software.Two crystallines,αB and βB1 crystallin,were identified using MALDI-TOF-MS.Conclusions Proteomic analysis of lens can be accomplished and the proteins can be well separated,moreover,differential proteins can be analyzed using 2-DE and mass spectrometry between diabetic cataract and age related cataract.These results indicate that αB and βB1 crystallin proteins accelerate the development of diabetic cataract.This technique offers a new avenue for clarity of lens proteins of diabetic cataract other than age related cataract.
