Up-regulation of prothymosin alpha in THP-1 cells infected with Mycobacterium tuberculosis.
- Author:
Ho Yeon SONG
1
;
Kwang Sik JANG
;
Hee Sun BYOUN
;
Shin Je LEE
;
Jin Koo KIM
;
Yong Kyung CHOE
;
Kwang Kjune KO
Author Information
1. Department of Microbiology, Soonchunhyang University College of Medicine, 366-1 Ssang Yong-dong, Chonan, Choong-Nam, 330-090, South Korea. songmic@asan.sch.ac.kr
- Publication Type:Original Article
- MeSH:
Animals;
Antigens, CD44;
Clone Cells;
Databases, Nucleic Acid;
Escherichia coli;
Eukaryotic Initiation Factor-2;
Ferritins;
Humans;
Macrophages;
Mice;
Mycobacterium bovis;
Mycobacterium tuberculosis*;
Mycobacterium*;
Phosphotransferases;
Receptors, Antigen, T-Cell;
Reverse Transcriptase Polymerase Chain Reaction;
Up-Regulation*
- From:Journal of the Korean Society for Microbiology
2000;35(2):149-157
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Mycobacterium tuberculosis is capable of growing and survival within macrophage. The purpose of this study was to identify the genes regulated by infection of mycobacteria in human monocytic THP-1 cells. We used the differential display reverse transcriptase polymerase chain reaction (DD RT-PCR) and nothern blot analysis to confirm the differentially expressed genes from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv, heat-kille Mycobacterium tuberculosis H37Rv and live Mycobacterium bovis BCG. Among many up or down-regulated clones, 27 clones were sequenced and compared with known genes on GenBank. Thirteen of over-expressed clones from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv were identical to human prothymosin alpha, eight were novel clones and six clones showed homology with Human ferritin H chain, Escherichia coli bgl, Mouse RNA-dependent EIF-2 alpha kinase, E. coli htrL, Hyaluronan receptor and T cell receptor. Our result suggests that Mycobacterium tuberculosis might regulate prothymosin alpha gene transcription in monocytic THP-1 cell.
