Application of HPLC in quantity of plasma rifampicin in patient taking simultaneously rifampicin - isoniazid - pyrazinamide
- Author:
Le Thi Luyen
- Publication Type:Journal Article
- Keywords:
HPLC method
- MeSH:
Chromatography, High Pressure Liquid;
lasma;
Pyrazinamide;
Isoniazid;
Rifampin
- From:Pharmaceutical Journal
2005;347(3):32-34
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
A HPLC method has been used to quantify plasma rifampicin and isoniazid and pyrazinamide simultaneously. Extraction of rifampicin in plasma samples was done as follows: mix 1ml of plasma containing rifampicin and 1.5 ml acetonitril on a vortex mixer for 1 minute prior to centrifugation at 3500 rpm for 30 minutes. The organic layer was filtered through 0.45m filter membrane and then 30l of this solution was injected into the HPLC system. The chromatographic conditions were as follows: in stationary phase: column: Apollo Alltech RP18 (250 x 4.6 mm; 5 m); in mobile phase: methanol - phosphate buffet solution containing 0.02M potassium dihydrogen phosphate adjusted to pH 4.5 by adding phosphoric acid (65: 35); Flow rate: 1.0 ml/min and UV detector: 254 nm
