Collagen type Ⅰ、Ⅵ and Matrix Metalloproteinase-2 in Normal and Keratoconus Corneas
- VernacularTitle:正常角膜与圆锥角膜中的Ⅰ型、Ⅵ型胶原与金属蛋白酶-2
- Author:
Lijun, ZHANG
;
Lixin, XIE
- Publication Type:Journal Article
- Keywords:
keratoconus;
collagen;
enzyme
- From:
Chinese Journal of Experimental Ophthalmology
2001;19(1):46-48
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo compare the levels and locations of metalloprotionase-2（MMP-2），collagen types Ⅰ and Ⅵ in keratoconus and normal human corneas．MethodsAn immunoperoxidase and indirect immunofluorescent technique were used to visualize the presence of collagen types Ⅰ， Ⅵ and MMP-2．The immunohistochemical staining was further analyzed and the intensity of reaction products was measured with an Image-processing system．ResultsCollagen type Ⅰ was demonstrated immunohistochemicall and indirect immunofluorescent technique in the bowman and stroma layers；collagen type Ⅵ was in epithelium basement，bowman，stroma and descement layers；MMP-2 was in epithelium，stroma and endothelium of all corneal sections．Compared with normal control specimens，the staining intensity of collagen types Ⅰ and Ⅵ was similar，while MMP-2 was markly increased．ConclusionsKeratoconus contained an increased level of MMP-2 and similar of collagen levels．These results lead further support to the hypothesis that degradation processes may be aberrant in keratoconus．
