Effects of subretinal fluid on proliferation of cultured human retinal pigment epithelial cells and fibroblast cells
- VernacularTitle:视网膜下液对培养的人眼视网膜色素上皮细胞和成纤维细胞增殖作用的影响
- Author:
Miaoqin, WU
;
Jing, WANG
- Publication Type:Journal Article
- Keywords:
proliferative;
vitreoretinopathy;
subretinal;
fluid;
retinal;
pigment;
epithelial;
cells;
fibroblast
- From:
Chinese Journal of Experimental Ophthalmology
2001;19(1):29-31
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo detect the effect of subretinal fluid （SRF） from different grades of proliferative vitreoretinopathy（PVR） on cultured retinal pigmental epithelial cells（RPE） and fibloblasts（FB）．MethodsThe effect of SRF from different grades of PVR on RPE and FB was determined using Brdu labeling technique and MTT assaying method，respectively．Results All samples showed a stimulating effect on the two kinds of cellular elements in a certain degree．At 1∶10 concentration，the cellular proliferation stimulating activity of SRF with PVR＜C1，PVR C1 and PVR＞C1 on RPE and FB was 128.5%，139.8%，156.8%and 126.3%，143.1%，172.3%of the control groups, respectively after 24 h stimulation.ConclusionsSRF’s proliferation-stimulating activity is correlated with the grades of PVR．
