DNA detection of human papilloma virus and herpes simplex virus in pterygia
10.3760/cma.j.issn.2095-0160.2011.03.013
- VernacularTitle:翼状胬肉中人乳头瘤病毒及单纯疱疹病毒的检测
- Author:
Ming, LU
;
Yuan, GAO
;
Qi, XIONG
- Publication Type:Journal Article
- Keywords:
Pterygium;
Human papilloma virus;
Herpes simplex virus;
Fluorescence quantitative PCR
- From:
Chinese Journal of Experimental Ophthalmology
2011;29(3):244-247
- CountryChina
- Language:Chinese
-
Abstract:
Background To explore the roles of human papilloma virus(HPV)and herpes simplex virus (HSV)in pathogenesis of pterygia are very important for provide more basis for the study of pterygia mechanisms.Objective The aim of the study was to detect the expression of HSV and HPV in pterygia. Methods A total of 68 specimens of pterygia and specimens of normal conjunction tissue were obtained during the operation.The expression of HSV DNA and HPV DNA in pterygia specimens and conjunetival tissue were detected and compared by real time fluorescence quantitative polymerase chain reaction(PCR).Written informed consent was obtained from each patient before the any medical procedure related to this study. Results Real time fluorescence quantitative PCR showed that HPV DNA was detected in 12(17.65％)pterygia specimens,and HSV DNA was found in 15 (22.06％)pterygia specimens；however them were detected in 6(8.82％)and 1(1.47％)in conjunctival tissue respectively.Significant differences were found in the expression both HSV DNA and HPV DNA between pterygium tissue and eonjunctival tissue(HPV：χ2=10.291,P<0.05；HSV：χ2=4.561,P<0.05).The co-expression of HSV DNA and HPV DNA was seen in 5(7.35％)pterygium specimens,but no any co-expression of HSV DNA and HPV DNA was in conjunctival specimen. Conclusion HSV and HPV may participate in the pathogenesis and development of pterygium.The detection of virus DNA can offer an experiment evidence of HSV and HPV in pterygium generation.
