Effects of ciliary neurotrophic factor mediated by ultrasound microbubbles intraocular transfer after optic nerve injury in rat

VernacularTitle:超声微泡介导CNTF基因眼内转染对视神经损伤大鼠作用的研究
Author: Min, LIU ; Su, LIU ; Zhi-gang, WANG ; Wen-yue, XIE
Publication Type:Journal Article
Keywords: Ultrasound microbubbles; Ciliary neurotrophic factor; Retinal ganglion cells; Gene transfeetion; Visual evoked potential
From: Chinese Journal of Experimental Ophthalmology 2011;29(4):303-307
CountryChina
Language:Chinese
Abstract: Background The key premise of genetic research and treatment is to select the desired gene vector,ultrasound microbubbles as a new type of gene vector can safe,fast and effectively enhance the gene transfection and expression by reversibility increasing the permeability of cells. Objective This study was to observe the effects of ciliary neurotrophic factor(CNTF)gene mediated by ultrasound microbubbles intraocular transfer on visual function and retinal ganglion cell(BGCs)after optic nerve injury. Methods Fifty-five adult Sprague-Dawley(SD)rats were divided into 6 groups randomly,including normal control group(n=5),sham injury group(n=10),simple injury group(n=10),naked plasmid group(n=10),plasmid+ultrasonic irradiation group(n=10)and ultrasound microbubbles group(n=10).The model of optic nerve injury Was made by forceps clip on the fight optic nerve.and the corresponding intervene was performed in different groups.Flash visual evoked potential(F