Adenylate cyclase stimulation and ocular hypertension inhibition by forskolin analogs
- VernacularTitle:佛司可林类似物激动腺苷酸环化酶及抑制兔高眼压探讨
- Author:
Weimin, YANG
;
Xinhua, LI
;
Zhihe, CHEN
;
Linghui, NIE
;
Boling, WANG
;
Zhiqiang, SHEN
- Publication Type:Journal Article
- Keywords:
forskolin;
isoforskolin;
deacetylforskolin;
1-acetylforskolin;
adenylate;
cyclase;
rabbit;
ocular;
hypertension
- From:
Chinese Journal of Experimental Ophthalmology
2001;19(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveForskolin （FSK） analogs，isoforskolin （isoF），deacetylforskolin（deaF），and 1-acetylforskolin（1-aF），extracted from Coleus forskohlii native to Yunnan，were assayed for their adenylate cyclase stimulating activities in vitro and for effects of two analogs on ocular hypertension （OHT） in water-loaded rabbits．MethodsAdenylate cyclase stimulation was determined by protein-binding method of radioimmunoassay，and intraocular pressure was monitored by pneumatonometer．ResultsIt showed that isoforskolin and forskolin stimulated adenylate cyclase in vitro with almost equal activity，deacetylforskolin with milder activity，and 1-acetylforskolin with little activity in vitro．1％ deaF and 1-aF suppressed rabbit OHT induced by water-loading for at least 3h，with the maximal inhibitory rates of 6.0，10.9% respectively．ConclusionThis study suggests that two foskolin analogs （isoforskolin，deacetylforskolin） possess adenylate cyclase stimulation activities in vitro；deacetylforskolin and 1-acetylforskolin suppress OHT induced by water-loading in rabbits．
