Comparative Analysis of Serological and Molecular Methods for the detection of Rice Grassy Stunt Virus
- VernacularTitle:水稻草矮病毒血清学和分子检测方法的比较
- Author:
Chun-mei, ZHANG
;
Qi-ying, LIN
;
Lian-hui, XIE
- Publication Type:Journal Article
- Keywords:
Rice grassy stunt virus;
serological detection;
molecular detection
- From:Virologica Sinica
2000;15(4):361-366
- CountryChina
- Language:Chinese
-
Abstract:
Methods of ELISA, nonradioactive molecular hybridiz ation and RT-PCR were applied in the detection of rice grassy stunt virus (RGSV ). The detection sensitivity of indirect ELISA using antiserum against fusion p rotein GST-NC was 1 mg of infected leaves or 84 ng of purified virus. The metho d of dot hybridization using NC, a DIG-labelled DNA probe was 50 μg diseased l e aves, or 6 ng purified preparations. The detection endpoint of RT-PCR was 10 μg diseased leaves, or 2 ng purified virus preparation. Comparisons of sensitivit y and maneuverability were made among these methods.
