Five Cytokines mRNA Expression in Rabbit Corneal Epithelium and Stroma
- VernacularTitle:五种生长因子mRNA在兔角膜中的表达
- Author:
Yisheng, ZHONG
;
Feng, CHENG
;
Kangsun, WANG
- Publication Type:Journal Article
- Keywords:
growth factorin situ hybridizationgene expressioncornearabbit
- From:Journal of Shanghai Jiaotong University(Medical Science)
2000;20(4):302-304
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression and distribution of epidermal growth factor (EGF), transforming growth factor-α(TGF-α), basic fibroblast growth factor (bFGF), transforming growth factor-β(TGF-β) and platelet-derived growth factor(PDGF) mRNA in the corneal epithelium and stroma of rabbit eyes, and evaluate their effects during corneal wound healing.MethodsThe expression and distribution of EGF, TGF-α, bFGF, TGF-β1 and PDGF mRNA in the corneal epithelium and stroma of rabbit eyes were detected by in situ hybridization.Results EGF,TGF-α and PDGF mRNA were only expressed in the normal corneal epithelium, but not in the corneal stroma. bFGF and TGF-β1 mRNA were expressed both in the normal corneal epithelium and stroma. ConclusionEGF, TGF-α, bFGF, TGF-β1 and PDGF can be excreted by cornea tissue,and regulate the corneal wound healing procedure.
