Sequencing and Homology Analysis for the Nucleocapsid Gene of JS/95/03 Strain of Avian Infectious Bronchitis Virus Isolated from Jiangsu, China
- VernacularTitle:传染性支气管炎病毒江苏分离株核蛋白基因序列测定与同源性分析
- Author:
De-sheng, CHEN
;
Jie-yan, PAN
;
Li-qun, JIA
;
Bao-xiang, CAI
;
Pu-yan N CHE
- Publication Type:Journal Article
- From:Virologica Sinica
2001;16(1):22-27
- CountryChina
- Language:Chinese
-
Abstract:
Field avian infectious bronchitis virus (IBV) designated as JS/9 5/03, which was isolated from Jiangsu province of china, was cultivated in chicken emb ryo. It's single strain RNA was extracted from purified virus and worked as temp late of reverse transcription polymerase chain reaction (RT-PCR), a pair of pri mer designed according to megalign results of published IBV sequences in Genbank was used to amplify the neucleocapsid gene, the RT-PCR product was sequenced d irectly. Sequence analysis revealed that the sequence of JS/95/03 is most homolo gized with that of M41 strain.
