Sequence Analysis of ORF3 and Partial ORF1 Region from Two Patients Infected with New Genotype of Hepatitis E Virus(HEV)
- VernacularTitle:新型戊肝病毒ORF3和部分ORF1基因的序列分析
- Author:
You-chun, WANG
;
Hua-yuan, ZHANG
;
Wen-jie, GU
;
Ling, ROGER
;
He-min, LI
;
Harrison J TIM
- Publication Type:Journal Article
- From:Virologica Sinica
2001;16(1):28-33
- CountryChina
- Language:Chinese
-
Abstract:
ORF3 and partial ORF1 regions were amplified with RT-PCR f rom two patients (T1 and T11)infected with new genotype of hepatitis E Virus. Th e PCR products were cloned and sequenced. The results showed that G-C rich regi on in ORF3 was deleted when amplified with normal PCR reaction. However, PCR rea ction containing G-C melt solution can overcome this problem. The sequence anal ysis showed that T1 and T11 belong to a new genotype of HEV which differs from g enotype I,II and III reported.T1 and T11 have 79%～82%, 80%～81% and 83%～85% id entical to genotype I,II and III respectively.
