Molecular Cloning and Sequencing of HCV E2 Gene
- VernacularTitle:HCV E2区基因的分子克隆及序列分析
- Author:
Dong-wei, ZHANG
;
Bu-feng, LIANG
;
Zi-bai, QI
;
Shi-Gan, LING
- Publication Type:Journal Article
- From:Virologica Sinica
2001;16(1):40-44
- CountryChina
- Language:Chinese
-
Abstract:
HCV RNA positive serum was first selected by RT-PCR test kit from several anti-HCV positive sera obtained from Xi'an.HCV RNA extrac ted from the elected sera was converted to cDNA by reverse transcription with ra ndom primer.Half-nested PCR was performed.The amplified product was 852 bp.The purified PCR product was digested by restriction endonucleases and then ligated to epressio vector pET-22b\++.Its nucleotide sequence was determined by dideoxy chain termination method.A comparison of the sequence with several isolates rep orted previously showed that the sequence belonged to HCV type Ⅱ.
