The Effect of Quercetin Targeted p53 Gene and Protein Expression in NB4 Cell Lines
- VernacularTitle:槲皮素对NB4细胞株p53及蛋白的影响
- Author:
Qing-wen, XIE
;
Jing-qiu, ZHAO
;
Zhi-wen, FANG
- Publication Type:Journal Article
- From:Journal of Shanghai Jiaotong University(Medical Science)
2001;21(1):8-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to study the value of drug treatment for leukemia, quercetin was delivered to the cultured NB4 cell line. MethodsUsing RT- PCR and western blot, we studied the expression of mutant p53 gene and protein after inducing 24, 48, 72, 96 and 120h by the quercetin with different concentrations (30, 60, 90μmol/L). Results We verified the results that quercetin with concentration of 30 ～90μmol/L could successfully inhibit the expression of mutant p53 protein, but the mutant p53 gene did not have. ConclusionThat data suggested that quercetin would be an effective method for the therapy of leukemia.
