A HPLC Method for Determination of the Content of an analog of Baogongteng A-ATST in the Aqueous Humor of Rabbits
- VernacularTitle:HPLC法测定兔眼房水中包甲素类似物一ATST的含量
- Author:
Li-min, YANG
;
Yi-fan, XIE
;
Hui-ning, WANG
- Publication Type:Journal Article
- From:Journal of Shanghai Jiaotong University(Medical Science)
2001;21(1):20-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective A HPLC method was established for determination of the content of ATST in the aqueous humor of rabbits. MethodsThe mobile phase was consisting of methanol-1％ triethylamine(57:43) and omeprazole (OMZ) as internal standard. The detection was carried out with an ultraviolet detector operated at 235nm. ResultsThere was linearity over the range of 2. 056～41.12 ug/ml in the humor aquosus, r=0.9997. The average recovery of ATST was 94.58 ％. Intra-day and in- ter-day RSD were less than 5 ％ and 10 ％ ( n = 5), respectively. Conclusion The method is reliable. It can be used for the study on the pharmacokinetics of ATST.
