The Biological Characterization of Bone Morphogenetic Protein 2 Gene Transfected Human Periodontal Ligament Fibroblasts
- VernacularTitle:骨形成蛋白2基因转染的人牙周膜成纤维细胞的生物学特性
- Author:
Xiao-hui, SI
;
Zheng, LIU
- Publication Type:Journal Article
- From:Journal of Shanghai Jiaotong University(Medical Science)
2001;21(1):24-27
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish the human periodontal ligament fibroblasts(HPDLFs)that express BMP2 and observe their biologicl characterization. MethodsA phagemid expression vector for BMP2 (pBK-B2) was transfected into HPDLFs by using LipofectAMINE. The BMP2 expression was determined by the immunohistochemical ABC method. The alkaline phosphatase (ALP) activity, osteocalcin (OC) production and capacity of mineralization were measured in the transfected cells. ResultsBMP2 protein was expressed in HPDLFs after gene transfection. The BMP2 gene transfected cells showed prominently elevated ALP activity, OC production and increase in mineralized nodules. ConclusionThe results indicate that BMP2 is expressed in HPDLFs and is involved in inducing differ- entiation of HPDLFs into osteoblast-like cells.
